The Response of a human bronchial epithelial cell line to histamine
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The Response of a human bronchial epithelial cell line to histamine intracellular calcium changes and extracellular release of inflammatory mediators by

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Published by U.S. Environmental Protection Agency in [Washington, D.C .
Written in English

Subjects:

  • Epithelial cells.,
  • Histamine.

Book details:

Edition Notes

StatementTerry L. Noah ... [et al.].
ContributionsNoah, Terry L., United States. Environmental Protection Agency.
The Physical Object
FormatMicroform
Pagination[9] p.
ID Numbers
Open LibraryOL14693302M

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  Histamine has a key role in allergic inflammatory conditions. The inflammatory responses resulting from the liberation of histamine have long been thought to be mediated by the histamine H 1 receptor, and H 1 -receptor antagonists — commonly known as antihistamines — have been used to treat allergies for many by: In order to study the effect of histamine on paracellular epithelial permeability and tight junctional integrity, we have cultured human bronchial epithelial cells to confluency and investigated the effect of topically applied microM histamine. Vignola AM, Campbell AM, Chanez P, et al. Activation by histamine of bronchial epithelial cells from nonasthmatic subjects. Am J Respir Cell Mol Biol ; 9: – Am J Respir Cell Mol Biol ; 9: – Using the human lung epithelial WI26VA4 cell line, we investigated the capacity of fenspiride, an anti-inflammatory drug with anti-bronchoconstrictor properties, to interfere with histamine-induced intracellular Ca 2+ increase and eicosanoid by: 3.

response to bacterial LPS in airway epithelial cells [16HBE14o- and primary nasal epithelial cells (PNECs)]. Methods Cell culture The immortalized bronchial epithelial cell line 16HBE14o-(obtained from D. Gruenert UCSF, USA) was grown as described previously (Kelly et al., c). PNECs were obtained from healthy volunteers (n = 7) and grown as Cited by:   Background: Airway epithelial cells form the first line of defense against B. cenocepacia infection. Results: Airway epithelial cells express low levels of caspase-1 and do not release processed IL-1β. Conclusion: Low level expression of caspase-1 results in the inability of airway epithelial cells to secrete IL-1β. Significance: This study provides novel insight into airway epithelial cell.   Co-culture of human eosinophils with BEAS-2B cells. The human bronchial epithelial cell line BEAS-2B cells were obtained from the American Type Culture Collections (Manassas, VA, USA) and Cited by: 5.   Optimally, a balanced Th1 and Th2 response is suited to the immune challenge, and a dysregulated response is linked to a variety of chronic inflammatory conditions like asthma and chronic bronchitis. 11 CD8+ T cells are mainly cytotoxic T cells. They secrete molecules that kill infected cells and tumor by:

Histamine is a biogenic amine with extensive effects on many cell types, including important immunologic cells, such as antigen-presenting cells, natural killer cells, epithelial cells. We found that air–liquid interface cultures of primary human bronchial epithelial cells (BECs) and an immortalized BEC model (Cdk4/hTERT HBEC) produce H 2O 2 in response to histamine. The main source of airway epithelial H 2O 2 is an NADPH dual oxidase, by: Cell Cultures-BET-lA, a human bronchial epithelial cell line transformed by the SV40 virus, and BEAS-2B, a human bronchial epithelial cell line transformed by an adenovirus SV40 hybrid virus (both gifts of J. E. Lechner, National Cancer Institute) (15) were cultured in serum-free LHC-9 medium with 25 pg/ml Fungizone. Southern blot analysis shows that stable integration of the transfected genes occurred. The cell line resembles morphologically the basal cells of the normal human bronchial epithelium, and is not tumorigenic in athymic nude mice. The cells are able to form tubules when grown in a basement membrane like matrix.